A Bioinspired And Chemically Defined Alternative To Dimethyl Sulfoxide For The Cryopreservation Of Human Hematopoietic Stem Cells

By Renata Gilfanova, Andrea Callegari, Adam Childs, Gaomai Yang, Miranda Luarca, Alan G. Gutierrez, Karla I. Medina, Justin Mai, Alvin Hui, Mark Kline, Xiaoxi Wei, Philip J. Norris and Marcus O. Muench

The cryopreservation of hematopoietic cells using dimethyl sulfoxide (DMSO) and serum is a common procedure used in transplantation. However, DMSO has clinical and biological side effects due to its toxicity, and serum introduces variation and safety risks. Explore the development of a novel class of ice-interactive cryoprotectants inspired by natural antifreeze proteins.

XT-Thrive is identified as a lead candidate to rival cryopreservation with 10% DMSO in serum based on post-thaw cell survival and short-term proliferation assays. Herein, novel cryoprotectants were tested on hematopoietic cells. The fully synthetic cryoprotectants control ice formation and are non-toxic, chemically stable, and are protein-free. Candidate formulations were compared to 10% DMSO in serum for use during liquid nitrogen storage (−196 °C).

Explore findings that show XT-Thrive can substitute the use of 10% DMSO and serum as a cryopreservation solution.

This article first appeared in Bone Marrow Transplantation.

Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/