INSIGHTS ON CELL & GENE MANUFACTURING

  • Where To Next With Continuous Processing?
    Where To Next With Continuous Processing?

    Today multiple suppliers offer commercial process solutions for continuous cell culture, continuous clarification, continuous chromatography, continuous virus inactivation and continuous filtration and their effectiveness is documented in the technical literature. So, what’s next? We have the tools; the technology is proven and early adopters are active.

  • Securing Bioprocess Film Supply
    Securing Bioprocess Film Supply

    Learn how a partnership built on technical expertise and trust is helping to secure the supply of a single-use film built for bioprocessing.

  • Closed And Semi-Automated Processing Of CAR T Cells
    Closed And Semi-Automated Processing Of CAR T Cells

    Challenges of T cell immunotherapies include the costly manufacturing process relying on lengthy and complex open workflows with high manual labor requirements that influence product variability. This application note describes the details of a robust CAR T cell manufacturing workflow that can be adapted for cGMP compliance in commercial production of CAR T cells.

  • Extractables Testing On Single-Use Connectors
    Extractables Testing On Single-Use Connectors

    Appropriating BPOG testing on single-use connectors to mitigate the risks of E&L, as demonstrated in this article, is crucial to delivering safe and effective drugs.

  • Comparing Continuous And Batch Processing In Downstream Purification
    Comparing Continuous And Batch Processing In Downstream Purification

    Current state-of-the-art continuous manufacturing technologies are being developed and implemented to manufacture a wide variety of products including monoclonal antibodies, recombinant proteins, and other biological modalities. Though upstream fed batch and perfusion bioreactors unit processes are relatively mature, downstream process unit operations are less mature. In this case study, Catalent compared the productivity of purifications running in batch versus continuous mode.

  • ICP-MS Analysis Of Multiple Trace Elements In Industrial Cell Lines
    ICP-MS Analysis Of Multiple Trace Elements In Industrial Cell Lines

    Characterization of trace elements is important for upstream process development and batch quality control. In this poster, Catalent Biologics outlines a strategy using inductively coupled plasma-mass spectrometry (ICP-MS) to conduct trace-level multiple analysis.

  • Study: One Novel Purification Tool And Its Effects On Host Cell Protein Reduction And Turbidity In Low Isoelectric Point Target Proteins
    Study: One Novel Purification Tool And Its Effects On Host Cell Protein Reduction And Turbidity In Low Isoelectric Point Target Proteins

    The 3M™ Emphaze™ AEX Hybrid Purifier is a novel purification tool that utilizes a Q-functional hydrogel and 0.2 μm membrane to reduce impurities in a biopharmaceutical downstream process. This study involved a Design of Experiment (DoE) approach to investigate the effects of pH and conductivity on the turbidity, yield, and impurity clearance of the Emphaze™ AEX Hybrid Purifier and the efficiency of the subsequent protein capture step. 

  • Achieving Unique Synergies In Antibody Expression
    Achieving Unique Synergies In Antibody Expression

    Shortening timelines to generate more productive, stable and high expressing cell lines is the goal of any cell line development program. This technical poster details the methodologies and clonal variation of combining a cell line development technology with a platform for clonal cell line selection.

  • Identifying Heterogeneity Within Rare Cell Populations By Pairing Single-Cell RNA-Seq With Cell Sorting
    Identifying Heterogeneity Within Rare Cell Populations By Pairing Single-Cell RNA-Seq With Cell Sorting

    Studying biology at a single-cell level can provide crucial insights into how individual cells contribute to human biology and disease. Here we demonstrate that the resolution of single-cell RNA sequencing (RNA-Seq) can be greatly enhanced by adding a cell sorting step prior to RNA-Seq analysis.

  • 10 Tips For Western Blot Detection Of Phosphorylation Events
    10 Tips For Western Blot Detection Of Phosphorylation Events

    The ability to interrogate protein phosphorylation events and aberrant phosphorylation levels is critical to our understanding of various biological and pathological processes, particularly in the research of diseases, like cancer. However, detection of phosphorylated proteins in western blots can be challenging. Following are ten tips to ensure robust data generation and cleaner blots.

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