Opto™ Cell Therapy Development Workflow: TCRseq Well Plate Kit

When using traditional techniques during cell therapy development, it is not possible to determine whether a specific cytokine-secreting cell can kill tumor targets or link this behavior directly to gene expression or genotype. This is particularly relevant in TCR-mediated therapies since the TCR sequence and associated affinity for antigen is directly linked to the degree of cytokine secretion and kinetics of target killing. 

Used downstream of functional T cell assays in the Opto™ Cell Therapy Development workflows, the TCRseq kit enables recovery of functionally validated T cell receptors (TCRs) from fewer than 10,000 input cells. Instead of re-expressing hundreds of TCRs in the hopes of identifying the rare sequence that imparts ideal specificity, the kit allows users to only sequence the T cells that matter.