Application Note

Integrated Pluripotent Aggregate Processing With The ekko™ System

ekko Acoustic Cell Processing System
Figure 1. ekko™ Acoustic Cell Processing System.

Background

Pluripotent stem cells (PSCs) and induced pluripotent stem cells (iPSCs) are promising sources for the next wave of cell therapies and regenerative medicine. As with many cell therapies, one major technical hurdle is the scalable production of PSCs for commercial applications, specifically when processing these cell types in aggregate form. Traditional technologies have not been able to deliver a closed and automated solution to gently process PSC aggregates that does not adversely impact cell viability, morphology, function, or efficacy.

Automated Media Exchange using the ekko™ System

Performing media exchanges throughout the expansion and/or diff erentiation processes of PSC cultures is generally a multi-step open process. Our ekko™ system enables automation of the media exchange and subsequent harvest steps. The system is a functionally closed, integrated manufacturing platform which gently processes cell aggregates and provides the option to remove any free single cells.

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