Hydrophobic Interaction Chromatography Screens For Downstream Process Development

Hydrophobic Interaction Chromatography (HIC) is a versatile purification technique used across many targets in the biopharma industry, from the polishing of monoclonal antibodies (mAb) and plasmid DNA (pDNA), to primary capture of more complex targets.

One of the key advantages of HIC is that the complex interactions between the hydrophobic regions on the target or the impurity can be exploited to achieve selectivity with both the ligand used on the adsorber and the strength and characteristics of the lyotropic salts used to “salt out” the target molecules.

A typical strategy in process development would be to screen ligands and conditions for a HIC purification step. Astrea Bioseparations has launched a HIC screening selection kit containing four different ligands to enable end users to screen their process. All four HIC resins can be produced at scale for full cGMP manufacturing implementation. This poster outlines a screening process for Butyl, Hexyl, Octyl and Phenyl ligands for several targets:

• mAb polishing step post-Protein A
• AAV 2 and AAV 9 capture from lysate
• pDNA polishing step post-AEX