Bruce Levine, Ph.D., the Barbara and Edward Netter Professor in Cancer Gene Therapy at the University of Pennsylvania, president-elect of the International Society for Cell and Gene Therapy, and Cell & Gene Editorial Advisory Board member, details seven cell and gene therapy trends to watch in 2020.
Application Note |
By Dianna Maar, Nicholas J. Heredia, and Shawn Hodges,Bio-Rad Laboratories, Inc.
Droplet Digital PCR (ddPCR) enables easy multiplexing of numerous targets within each fluorescent channel. Amplitude-based multiplexing achieves this by varying primer and/or probe concentrations. In probe-mixing multiplexing, probes are mixed at desired concentrations to place targets in a defined position on a 2D plot. This paper describes strategies for both approaches using the large portfolio of ddPCR assays readily available through Bio Rad.
The viral vector market is highly active, and the interest in production technologies is driven by recent approvals in cell and gene therapy. Here, the challenges in viral vector manufacturing are shared so that a scalable and cost-efficient process suitable for GMP manufacturing environments can be obtained.
This paper is intended as a discussion of the commonly encountered hurdles in studying rare diseases and how a cohesive and holistic approach can mitigate issues and help bring new treatments to patients.